Abstract: The extraction in polysaccharide in Houttuynia cordata was studied.The results showed that the best extraction conditions were:heating circumfluence for 2 h and for 3 times,the ratio of water to solid was 30:1.The samples were precipitated by ethanol to remove protein and then separated by gel chromatography.The samples were extracted with water by ultrasonic oscillation and by solid phase extraction with Sep-pak C18 cartridge.Sugars were separatedusing Waters high performance carbohydrate column as solid phase,7:3 with acetonitrile:water as mobile phase,and detected with evaporative light-scatter detector.The component of polysaccharides of Houttuynia cordata was m(wood sugar):m(fructose):m(arabinose):m(galactose)=2.209:1.587:1.000:2.092.