Abstract: A series of phenol molecular imprinted polymers(MIP1-17)were prepared with a new functional monomer 1-phenyl-3-methyl-4-methacryloyl-5-pyrazolone(PMMP)by orthogonal design table of L16(45)considering 3 factors:the kind of porogen solvent,the dosage of functional monomer and that of cross linker.The optimum molecular imprinted polymer(MIP17)was furthermore used in a mixed standard sample to study its selectivity.And the separation of a real phenol-contained water system was also investigated with this MIP17 solid phase extraction column.The results showed that MIP17 prepared using PMMP as functional monomer showed better selectivity,indicating a certain potential application in separation and enrichment of phenol.