Abstract: The microsporogenesis,megasporogensis and the formation of male gametophyte and female gametophyte in Mahonia duclouxiana were first reported.The main results can be concluded as follows: anthers are tetrasporangiate; the wall development is dicotyledonous type; glandular binucleate tapetum; endothecium of the chamber develops characteristic banded thickening during the maturation.Cytokinesis of meiosis in microsporocyte is successive and the tetrads are biaxial symmetrical.Pollen grains are mainly 2-cell type when shedding.Solitary pistil is monocarpellary with 4-6 ovules,marginal placenta,crassinucellate with double integument.Micropyle is formed in both inner and outer integument,shaped as "Z"; single archesporial cell locates under nucellar epidermis; the tetrad of megaspores is linear,the first cell from the chalazal end develops functional megaspore,the development of embryo sac is Polygonum type.