赵榕, 钱正强, 王焕冲, 徐丰磊, 宫霞, 陈姝, 杨明挚. 昆明西山野生蘡薁葡萄资源及其遗传多样性分析[J]. 云南大学学报(自然科学版), 2010, 32(6): 710-714, .
引用本文: 赵榕, 钱正强, 王焕冲, 徐丰磊, 宫霞, 陈姝, 杨明挚. 昆明西山野生蘡薁葡萄资源及其遗传多样性分析[J]. 云南大学学报(自然科学版), 2010, 32(6): 710-714, .
ZHAO Rong, QIAN Zheng-qiang, WANG Huan-chong, XU Feng-lei, GONG Xia, CHEN Shu, YANG Ming-zhi. A study on the distribution and genetic diversities of wild graperesources Vitis bryoniaegolia in Xishan mountain,Kunming[J]. Journal of Yunnan University: Natural Sciences Edition, 2010, 32(6): 710-714, .
Citation: ZHAO Rong, QIAN Zheng-qiang, WANG Huan-chong, XU Feng-lei, GONG Xia, CHEN Shu, YANG Ming-zhi. A study on the distribution and genetic diversities of wild graperesources Vitis bryoniaegolia in Xishan mountain,Kunming[J]. Journal of Yunnan University: Natural Sciences Edition, 2010, 32(6): 710-714, .

昆明西山野生蘡薁葡萄资源及其遗传多样性分析

A study on the distribution and genetic diversities of wild graperesources Vitis bryoniaegolia in Xishan mountain,Kunming

  • 摘要: 调查了昆明西山野生蘡薁葡萄(Vitis bryoniaegolia Bge)的分布特征,并利用RAPD分子标记对其5个群体的遗传多样性进行了分析.采用5个RAPD引物扩增,共产生71条DNA条带,其中多态条带68条.在物种水平上,蘡薁葡萄多态位点百分率(PPB)为95.77%,Nei's基因多样性指数(H)为0.3442,Shannon's多样性信息指数(Hsp)为0.5100;在群体水平上,PPB差异较大(35.21%~84.51%),平均值为60.85%,H平均为0.2234,Ho平均为0.3305.各群体间的Nei's遗传一致度(I)范围为0.7171~0.9489.基于Nei's遗传多样性分析得出的群体间遗传分化系数Gst=0.3269,表明西山蘡薁葡萄的遗传多样性主要来源与群体内而不是群体间.Mantel检测发现,群体间的遗传距离和物理距离之间呈显著的正相关(r=0.981,P=0.0190.05).

     

    Abstract: The ecological distribution characters of Vitis bryoniaeglia Beg from Xishan mountain in Kunming were investigated,the genetic diversities based on the RAPD markers were studied in 5 groups of Vitis bryoniaegolia Bge.The results indicated that 68 diversified loci in total 71 DNA bands were amplified by using 5 random primers.At species level,the percentage of polymorphic bands (PPB), Nei's gene diversity (H) and the Shannon's information index ( Hsp ) were 95.77%,0.3442 and 0.5100,respectively.While at groups level ,the average PPB was 60.85 % (varied from 35.21 %—84.51%).The average H and Ho were 0.2234 and 0.3305,respectively.The coefficient of genes differentiation among groups ( Gst ) based on Nei's genetic distance was 0.3269,which implied that the gene variation was originated mainly within the groups (77.31%) other than among (32.69%) the groups.Genetic identity (I) of the 5 groups was varied from 0.7171 to 0.9489.The geographical distance was significant correlated with the genetic distance based by the Mantel analysis as r=0.981,P=0.0190.05.

     

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