Abstract: It is studied that the different extraction method of total flavonoids from Artemisia Rupestris L.using the orthogonal method to develop an optimal process.Based on extraction ratio as standard,reflux method is the best ,the net equity extraction of flavonoids reached 47.8mg/g,followed by microwave-assisted method,the net equity extraction of flavonoids reached 42.13mg/g,the lowest is the ultrasonic method,the net equity extraction of flavonoids is just 38.89mg/g.For the extraction efficiency,microwave extraction is the optimal method,followed by ultrasonic extraction,reflux method has the minimum efficiency.