Abstract: The anti-TMV monoclonal antibodies were obtained through monoclonal antibody technology.The rapid test method of tobacco mosaic virus was established through the integration of colloidal gold labeling,immunoassay and quantitative determination of SQUID magnetic technology.The colloidal gold test strips were established for qualitative detection of TMV and the detection sensitivity was 1ng/mL.The magnetic immunoassay strips were established for quantitative detection of TMV and the detection sensitivity was 0.1ng/mL.Both methods were simple,rapid,high sensitivity and specificity.It's feasible to the rapid on-site screening of the TMV disease and disease-free tobacco plants for tobacco seedlings.