Abstract: Samples were taken from the three different populations of Coptis teeta in Genuine Producing Areas.Comparison and analysis of the individual biomass (rhizome,fibrous root,leaf) and the individual key alkaloid contents (berberine,jateorhizine,palmatine) were performed.The results suggested that there were individual differences of biomass and alkaloid contents in the samples from three different populations,the most stable of biomass and alkaloid contents was detected in rhizome,the stable of biomass and alkaloid contents was found in leaf and the large difference of biomass and alkaloid contents was detected in fibrous root.The key alkaloid contents in leaf and fibrous root,which were unconventional medicinal parts,were one third in whole plant,and alkaloid in leaf and fibrous root can be used.The biomass and alkaloids in individual plant presents significantly positive correlation.