Abstract: A method that could roundly express the total differences of the chemical components of tobacco varieties was reported based on UV-VISspectroscopy.With referring pattern recognition technology,the spectral features to identify and distinguish the Hongda,K326 and Yun series varieties were extracted.The results showed that:(a) five feature waves ranged at 800—791,787—778,676—657,527—518 nm and 295—286 nm with 60 nm long could express the total features of Hongda different from K326 and Yun series.(b) five feature wave ranged 792—783,666—657,620—611,551—542 nm and 365—346 nm with 60 nm long could express the total differences of K326 from Hongda and Yun series;(c) six feature waves ranged 710—701,676—657,574—565,375—366,335—326 nm and 285—276 nm with 70 nm long could express the total differences of Yun series refered to Hongda and K326.