伍红, 秦天莺, 谭德勇, 农向. 瑞氏木霉QM9414利用蔗渣发酵产纤维素酶的研究[J]. 云南大学学报(自然科学版), 2008, 30(6): 620-624,640.
引用本文: 伍红, 秦天莺, 谭德勇, 农向. 瑞氏木霉QM9414利用蔗渣发酵产纤维素酶的研究[J]. 云南大学学报(自然科学版), 2008, 30(6): 620-624,640.
WU Hong, QIN Tian-ying, TAN De-yong, NONG Xiang. Study on production of cellulase using bagasse by Trichoderma reesei QM9414[J]. Journal of Yunnan University: Natural Sciences Edition, 2008, 30(6): 620-624,640.
Citation: WU Hong, QIN Tian-ying, TAN De-yong, NONG Xiang. Study on production of cellulase using bagasse by Trichoderma reesei QM9414[J]. Journal of Yunnan University: Natural Sciences Edition, 2008, 30(6): 620-624,640.

瑞氏木霉QM9414利用蔗渣发酵产纤维素酶的研究

Study on production of cellulase using bagasse by Trichoderma reesei QM9414

  • 摘要: 以蔗渣为培养基料,通过单因子及正交试验,对瑞氏木霉QM9414固体发酵产纤维素酶的产酶条件进行了探讨.其优化的产酶条件为:甘蔗渣2.5 g,麸皮1 g,加含7.5 g/L(NH4)2SO4的Mandels营养液14 mL(干物质(g)与水(mL)的比例为1:4),调初始pH 4.0,30℃发酵120 h.在此优化条件下,每克干曲产纤维素酶酶活力可达8.26 U.

     

    Abstract: Utilizing bagasse as substrate the culture conditions for therma reesei QM9414 was studied through single factor experiments and orthogonal test.The results showed that the optimal culture conditions were as follows:medium prepared from 1g bagasse,2.5 g wheat bran and 14mL Mandels nutritional liquid containing 7.5 g/L(NH4)2SO4,adjustment of initial pH to 4.0,at 30℃ for 120h.Under these optimal fermentation conditions,cellulase activity reached 8.26 U/g dry medium.

     

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