张伟, 闫娟娟, 杨兆祥. pH色谱法测定灯盏花乙素离解常数[J]. 云南大学学报(自然科学版), 2010, 32(2): 196-200 .
引用本文: 张伟, 闫娟娟, 杨兆祥. pH色谱法测定灯盏花乙素离解常数[J]. 云南大学学报(自然科学版), 2010, 32(2): 196-200 .
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Determination of dissociation constant of scutellarin by thin-layer pH-chromatography and HPLC pH-chromatography[J]. Journal of Yunnan University: Natural Sciences Edition, 2010, 32(2): 196-200 .
Citation: Determination of dissociation constant of scutellarin by thin-layer pH-chromatography and HPLC pH-chromatography[J]. Journal of Yunnan University: Natural Sciences Edition, 2010, 32(2): 196-200 .
shu

pH色谱法测定灯盏花乙素离解常数

Determination of dissociation constant of scutellarin by thin-layer pH-chromatography and HPLC pH-chromatography

    摘要
  • 摘要: 通过高效液相色谱(HPLC)和薄层色谱法(TLC)对灯盏花乙素离解常数进行测定方法研究.在薄层色谱方法中,利用待测组分比移值(Rf值)与固定相pH值的Rf-pH曲线方程;在高效液相色谱方法中,利用待测组分保留因子(κ值)与流动相pH值的κ-pH曲线方程,测定了灯盏花乙素的离解常数,并比较了HPLC和TLC法对灯盏花乙素离解常数的测定.HPLC与TLC法对灯盏花乙素离解常数的测定中κ-pH曲线和Rf-pH曲线均符合S型数理模型,该文首次报道了灯盏花乙素离解常数及测定方法.用所建立的方法分别测定了灯盏花乙素的离解常数并进行了比较,HPLC法准确度高,重现性好,方便简单,分析快速、准确.

     

    Abstract: Objective:To determine the dissociation constant of scutellarin by thin-layer pH-chromatography and HPLCpH-chromatography.Method:The dissociation constant of scutellarin is calculated respectively from the data of Rf-pHcurves in the thin-layer pH-chromatography,and the retention factors-pH of mobile phase in the HPLCpH-chromatography.Results:The curves of the two methods showed to be suitable for sigmoidal fit.The dissociation constant of scutellarin and its determination were studied for the first time.Conclusion:Compared the thin-layer pH-chromatography with the HPLCpH-chromatography,the method of HPLCph-chromatography is more repetitive,rapid and accurate.

     

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