Abstract: The distribution and species diversity of nematode-trapping fungi(NTF) in soils of eucalyptus were investigated.Totally,18 species falling into 4 genera of NTF were identified from 420 soil samples of eucalyptus in Wenshan,Yunnan,collected in 2004 and 2005.The abundant species were Arthrobotrys thaumasium and A.oligospora with the highest occurring frequencies as 25.40% and 21.21% respectively.During the 2 years,eucalyptus planting was not found to affect negatively on the species diversity of NTF,but easily reduced the contents of N,P,K and organic matter of soil.In eucalyptus plantation,NTF showed high community similarity in adhering regions but more change was found in different sampling time for the fungal community in same place.Diversity of NTF was positively correlated with the contents of N,P,K and organic matter.