尹丽珠, 张学忠, 冯雷, 珠娜, 祝红昆, 牛之瑞. 超高效液相色谱-串联质谱法检测禽畜肉中的氯霉素残留[J]. 云南大学学报(自然科学版), 2011, 33(3): 336-339 .
引用本文: 尹丽珠, 张学忠, 冯雷, 珠娜, 祝红昆, 牛之瑞. 超高效液相色谱-串联质谱法检测禽畜肉中的氯霉素残留[J]. 云南大学学报(自然科学版), 2011, 33(3): 336-339 .
shu
YIN Li-zhu, ZHANG Xue-zhong, FENG Lei, ZHU Hong-kun, ZHU Na, NIU Zhi-rui. Determination of chloramphenicol residues in meat by UPLC-MS/MS[J]. Journal of Yunnan University: Natural Sciences Edition, 2011, 33(3): 336-339 .
Citation: YIN Li-zhu, ZHANG Xue-zhong, FENG Lei, ZHU Hong-kun, ZHU Na, NIU Zhi-rui. Determination of chloramphenicol residues in meat by UPLC-MS/MS[J]. Journal of Yunnan University: Natural Sciences Edition, 2011, 33(3): 336-339 .
shu

超高效液相色谱-串联质谱法检测禽畜肉中的氯霉素残留

Determination of chloramphenicol residues in meat by UPLC-MS/MS

    摘要
  • 摘要: 研究了一种针对禽畜肉中氯霉素残留量的超高效液相色谱-串联质谱联用分析方法.本方法采用乙酸乙酯萃取禽畜肉中的氯霉素,提取液氮吹至干后用超纯水溶解样品残渣,然后用HLB小柱进行固相萃取,经过净化的样品通过超高效液相色谱进行分离,然后用电喷雾质谱负离子多反应监测模式对氯霉素进行检测.该方法的检出限为0.0025μg/kg,添加水平为0.1~5.0μg/kg时,加标回收率为75.8%~82.5%,相对标准偏差小于10%.该方法具有灵敏度高、选择性好、分析时间短等优点.

     

    Abstract: A new determination of chloramphenicol in meat was introduced.Solid-phase extraction (SPE) was applied in pretreatment of samples which were separated by ultra performance liquid chromatography (UPLC) with acetronitrile-water as mobile phase afterwards.The detection of chloramphenicol residues was performed by MS/MS in ESI(-)-MRM mode.The results shows that the pretreatment,as well as the analytical approach, was highly efficient, sensitive and also specific to chloramphenicol.

     

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