钟莲梅, 宗一, 戴纪男, 杨萍, 张伟, 詹东, 陆地, 孙俊. 元宝枫叶黄酮抑制脂多糖诱导的小胶质细胞激活的作用[J]. 云南大学学报(自然科学版), 2011, 33(3): 345-349, .
引用本文: 钟莲梅, 宗一, 戴纪男, 杨萍, 张伟, 詹东, 陆地, 孙俊. 元宝枫叶黄酮抑制脂多糖诱导的小胶质细胞激活的作用[J]. 云南大学学报(自然科学版), 2011, 33(3): 345-349, .
Attenuated LPS-induced activation of microglia byflavonoids from the leaves of Acer truncatum[J]. Journal of Yunnan University: Natural Sciences Edition, 2011, 33(3): 345-349, .
Citation: Attenuated LPS-induced activation of microglia byflavonoids from the leaves of Acer truncatum[J]. Journal of Yunnan University: Natural Sciences Edition, 2011, 33(3): 345-349, .

元宝枫叶黄酮抑制脂多糖诱导的小胶质细胞激活的作用

Attenuated LPS-induced activation of microglia byflavonoids from the leaves of Acer truncatum

  • 摘要: 探讨枫叶黄酮对脂多糖(Lipopolysaccharide,LPS)诱导的小胶质细胞株BV-2细胞炎性因子释放的抑制作用.用LPS刺激BV-2细胞构建炎症模型,采用免疫荧光双标和RT-PCR方法检测不同浓度枫叶黄酮(5,10,15μmol/L)对细胞炎性蛋白酶诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和环氧合酶-2 (cyclooxygenase-2,COX-2)、细胞炎性因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素-1β (interleukin-1β,IL-1β)、炎性信号蛋白核因子-κB(nuclear factor-κB,NF-κB)蛋白与mRNA的表达变化.结果发现:不同浓度的枫叶黄酮在翻译水平和转录水平上明显抑制了LPS诱导的细胞炎性蛋白酶iNOS和COX-2,细胞炎性因子TNF-α和IL-1β与炎性信号蛋白NF-κB的上调.上述结果表明枫叶黄酮可通过调控LPS诱导的小胶质细胞株BV-2细胞炎性因子释放从而抑制小胶质细胞激活,发挥抗神经炎症的作用.

     

    Abstract: To investigate suppressing effects of flavonoids from the leaves of Acer truncatum in the activation od BV-2 microglia,cells were treated with flavonoid (5,10 ,15μmol/L) prior to LPS exposure,then the effects on the mRNA and protein levels of pro-inflammatory enzymes,inducible nitric oxide synthase (iNOS),cyclooxygenase-2 (COX-2),pro-inflammatory cytokines,tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β) and inflammatory signaling proteins nuclear factor-κB (NF-κB) were analysed by RT-PCR and double-immunofluorescence labeling assay.The results indicate that flavonoid has an effect on the expression of iNOS,COX-2,TNF-α,IL-1β and NF-κB through transcriptional and translational inhibition.Therefore we think the flavonoid can restrain microglial cells activity by means of regulation pro-inflammatory mediators of BV-2 cells that induced by LPS,so it can exert anti-neuroinflammatory actions.

     

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