运用链特异性RT-PCR法进行脊髓灰质炎灭活疫苗的灭活验证

Detection of inactivated polio virus vaccines by strand specific RT-PCR

摘要: 运用链特异性逆转录聚合酶链式反应法(RT-PCR)建立了一种快速、灵敏、特异的脊髓灰质炎灭活疫苗(IPV)的灭活验证体系.以Sabin株脊髓灰质炎病毒悬液作为阳性对照,对脊髓灰质炎灭活疫苗进行检测.结果表明,链特异性RT-PCR法对具有活性的脊髓灰质炎病毒检测为阳性,而对脊髓灰质炎灭活疫苗的检测结果为阴性.链特异性RT-PCR法可作为一种简便、快速、灵敏的脊髓灰质炎灭活疫苗灭活验证方法,大大缩短了检测周期,在脊髓灰质炎灭活疫苗灭活验证的常规检测中具有较好的实际应用价值.

Abstract: The study was to find a rapid and sensitive methodology to test the effectiveness of inactivation of polio virus vaccine (IPV).Strand specific reverse transcriptase-polymerase chain reaction (RT-PCR) was applied to detect polio viruses in IPV.Sabin strains of polio virus were used in control experiments.It was proved that strand specific RT-PCRwas a sensitive and accurate method in rapid test of the effectiveness of inactivated virus polio vaccines.The method produced final results within 4—5 days,in contrast,the conventional method took about 35 days.This method was quite useful in the vaccine safety of IPVs.