阳春砂仁药材质量标准研究
Studies on quality contral standard of Amomum villosum
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摘要: 建立阳春砂仁含量测定方法.采用气相色谱(GC)法测定阳春砂仁中乙酸龙脑酯的含量,采用高效液相色谱(HPLC)法测定阳春砂仁中槲皮苷的含量.乙酸龙脑酯进样量在0.1271~2.5424μg(r=0.99908)范围内与峰面积呈良好的线性关系,平均回收率为97.95%,相对标准偏差为2.08%(n=6);槲皮苷进样量在0.006~0.013μg(r=0.99956)范围内与峰面积呈良好的线性关系,平均回收率为103.73%,相对标准偏差为0.80%(n=6).本方法简便,快速准确,可作为阳春砂仁含量测定质量控制方法.Abstract: To establish methods for the determination of the quality of Amomum villosum.GC was adopted for the determination of bornyl acetae in Amomum villosum,HPLC was adopted for the determination of quercitroside in Amomum villosum.There was a good liner relationships between the peak areas and the sample contents within the ranges of 0.127 1-2.542 4 μg (r=0.99908) and 0.006-0.013 μg (r=0.99956)for bornyl acetae and quercitroside respectively.The average recovery rates of bornyl acetae and quercitroside were 97.95% (n=6,RSD=2.08%) and 103.73% (n=6,RSD=0.80%),respectively.The methods are simple,rapid and accurate and can be used for quality control of Amomum villosum.
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