Abstract: To study the chemical constituents of Skimmia arborescens.The chemical constituents were isolated by silica gel column chromatography,Pharmadex LH-20,RP-Csub>18,and the 1H NMR,1C NMR spectroscopic analysis were employed for the structural elucidation.Six compounds were isolated from Skimmia arborescens.Their structures were elucidated as quercetin(1),quercetin 3-O-α-L-Rha(2),kaempferol-3,7-o-β-D-glucosides(3),poncirin(4),hesperidin(5),1-methyl-2(Z)-7-phenyl-4(1H)-quinolone(6),The 6 compounds were isolated from the plant for the first time.Using acetic acid writhing and hot plate test in mice,the pain releasing effects of the total extract and the total alkaloids from the plant were evaluated.The results indicated that both the total extract and total alkaloids of Skimmia arborescens could markedly decrease mice writhing caused by acetic acid.Furthermore,the total alkaloid of Skimmia arborescens could extend evidently pain threshold in mice.