Abstract:
To investigate suppressing effects of 17β-Estradiol(17β-E2)in the activation of Raw 264.7 cells,the cells were treated with 17β-E2 prior to Homocystine(Hcy)exposure,then the effects on the mRNA and protein levels of pro-inflammatory enzymes,cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS),and pro-inflammatory cytokines,tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β),inflammatory signaling proteins nuclear factor-κB(NF-κB)were analysed by reverse transcription-polymerase chain reaction(RT-PCR)and double-immunofluorescence labeling assay,and the effects of 17β-E2 on viability of Raw 264.7 cells were measured by MTT assay.The results showed that 17β-E2 had an effect on COX-2,iNOS,TNF-α,IL-1β,NF-κB protein and mRNA expression levels.Arising from the above,we think 17β-E2 can restrain osteoclasts activity by means of regulation Hcy-induced pro-inflammatory mediators in Raw 264.7 cells,and exert its anti-osteoporosis actions.