何颖, 陈学秋, 宗一, 詹东, 杨萍, 刘世昌, 陆地, 桂莉. 17β-雌二醇抑制高同型半胱氨酸诱导破骨细胞Raw 264.7激活的作用研究[J]. 云南大学学报(自然科学版), 2011, 33(6): 716-722.
引用本文: 何颖, 陈学秋, 宗一, 詹东, 杨萍, 刘世昌, 陆地, 桂莉. 17β-雌二醇抑制高同型半胱氨酸诱导破骨细胞Raw 264.7激活的作用研究[J]. 云南大学学报(自然科学版), 2011, 33(6): 716-722.
HE Ying, CHENG Xue-qiu, ZONG Yi, ZHAN Dong, YANG Ping, LIU Shi-chang, LU Di, GUI Li. The effects of 17β-estradiol attenuated homocystine-induced activation of Raw 264.7 cells[J]. Journal of Yunnan University: Natural Sciences Edition, 2011, 33(6): 716-722.
Citation: HE Ying, CHENG Xue-qiu, ZONG Yi, ZHAN Dong, YANG Ping, LIU Shi-chang, LU Di, GUI Li. The effects of 17β-estradiol attenuated homocystine-induced activation of Raw 264.7 cells[J]. Journal of Yunnan University: Natural Sciences Edition, 2011, 33(6): 716-722.

17β-雌二醇抑制高同型半胱氨酸诱导破骨细胞Raw 264.7激活的作用研究

The effects of 17β-estradiol attenuated homocystine-induced activation of Raw 264.7 cells

  • 摘要: 探讨17-β雌二醇(17β-Estradiol,17β-E2)对高同型半胱氨酸(High Homocystine,HHcy)诱导的破骨前体细胞株Raw 264.7炎性因子释放的抑制作用.用同型半胱氨酸(Homocystine,Hcy)刺激Raw264.7细胞构建炎症模型,采用四甲基偶氮唑蓝比色法(MTT)检测17β-E2对Raw 264.7细胞的活力影响,免疫荧光双标和RT-PCR方法检测不同浓度17β-E2(1,10 nmol/L和1μmol/L)对环氧合酶-2(COX-2)和细胞炎性蛋白酶诱导型一氧化氮合酶(iNOS)、细胞炎性因子肿瘤坏死因子-α(TNF-α)和白介素-1β(IL-1β)、炎性信号蛋白核因子-κB(NF-κB)蛋白与mRNA的表达变化.结果发现:不同浓度的17β-E2在翻译水平和转录水平上明显抑制了Hcy诱导的细胞炎性蛋白酶COX-2和iNOS,细胞炎性因子TNF-α和IL-1β与炎性信号蛋白NF-κB的上调,并且COX-2和IL-1β蛋白和mRNA的表达呈剂量依赖性.上述结果表明17β-E2可通过调控Hcy诱导的破骨前体细胞株Raw264.7细胞炎性因子释放从而抑制破骨激活,发挥抗骨质疏松的作用.

     

    Abstract: To investigate suppressing effects of 17β-Estradiol(17β-E2)in the activation of Raw 264.7 cells,the cells were treated with 17β-E2 prior to Homocystine(Hcy)exposure,then the effects on the mRNA and protein levels of pro-inflammatory enzymes,cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS),and pro-inflammatory cytokines,tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β),inflammatory signaling proteins nuclear factor-κB(NF-κB)were analysed by reverse transcription-polymerase chain reaction(RT-PCR)and double-immunofluorescence labeling assay,and the effects of 17β-E2 on viability of Raw 264.7 cells were measured by MTT assay.The results showed that 17β-E2 had an effect on COX-2,iNOS,TNF-α,IL-1β,NF-κB protein and mRNA expression levels.Arising from the above,we think 17β-E2 can restrain osteoclasts activity by means of regulation Hcy-induced pro-inflammatory mediators in Raw 264.7 cells,and exert its anti-osteoporosis actions.

     

/

返回文章
返回