Abstract:
By using M13 phage display techniques,12-mer peptide ligands that bind specifically to HPV 58 E7 protein were isolated,and then we compared all the sequences to get consensus sequences,moreover,through BLASTP endogenous proteins which probably interact with E7 protein were found.Using GET-E7 fusion protein as a positive screening target and GST protein as a negative one,we took four rounds of negative-positive selection,after that,71 positive clones were selected.By DNA sequencing and sequence analysis,we got two consensus sequences:NHXXANPQQXPQ and TMGFTAPRFPHY.We analyzed the homologous proteins of the 71 peptides by BLASTP,which will be very helpful in the study of E7 carcinogenesis.