范久松, 赵洪彬, 林洁, 马岚. HPV 58型E7蛋白特异结合多肽的筛选及分析[J]. 云南大学学报(自然科学版), 2011, 33(5): 615-620.
引用本文: 范久松, 赵洪彬, 林洁, 马岚. HPV 58型E7蛋白特异结合多肽的筛选及分析[J]. 云南大学学报(自然科学版), 2011, 33(5): 615-620.
FAN Jiu-song, ZHAO Hong-bin, LIN Jie, MA Lan. Screening and analysis peptides that bind specifically to HPV 58 E7 protein[J]. Journal of Yunnan University: Natural Sciences Edition, 2011, 33(5): 615-620.
Citation: FAN Jiu-song, ZHAO Hong-bin, LIN Jie, MA Lan. Screening and analysis peptides that bind specifically to HPV 58 E7 protein[J]. Journal of Yunnan University: Natural Sciences Edition, 2011, 33(5): 615-620.

HPV 58型E7蛋白特异结合多肽的筛选及分析

Screening and analysis peptides that bind specifically to HPV 58 E7 protein

  • 摘要: 利用M13噬菌体展示技术筛选HPV 58型E7蛋白特异性结合12肽,对这些多肽的序列比对分析得出其共有序列,同时通过BLASTP序列对比分析获得能与E7结合的内源蛋白.利用GST-E7融合表达蛋白为正筛靶分子,GST蛋白为负筛靶分子进行4轮的正负亲和筛选,经过ELISA活性鉴定获得71株阳性克隆,通过DNA测序和序列分析,得到2个能与E7蛋白发生特异性结合的共有序列NHXXANPQQXPQ和TMGFTAPRF-PHY.通过BLASTP分析所有71个多肽在体内的同源蛋白,它们能为对E7蛋白的致癌机理研究提供方向.

     

    Abstract: By using M13 phage display techniques,12-mer peptide ligands that bind specifically to HPV 58 E7 protein were isolated,and then we compared all the sequences to get consensus sequences,moreover,through BLASTP endogenous proteins which probably interact with E7 protein were found.Using GET-E7 fusion protein as a positive screening target and GST protein as a negative one,we took four rounds of negative-positive selection,after that,71 positive clones were selected.By DNA sequencing and sequence analysis,we got two consensus sequences:NHXXANPQQXPQ and TMGFTAPRFPHY.We analyzed the homologous proteins of the 71 peptides by BLASTP,which will be very helpful in the study of E7 carcinogenesis.

     

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