CDV,CPV,CAV三联DNA疫苗的实验免疫研究

Research on the nucleic acid vaccine and immunity of combine CDV,CPV and CAV

摘要: 犬瘟热病毒(CDV)、犬细小病毒(CPV)、犬腺病毒(CAV)是引起犬传染病的主要病原体,严重危害犬的健康,基因疫苗具有安全和克服幼犬母源抗体对主动免疫干扰的优点.根据CDV的H基因、CPV的VP1基因、犬2型腺病毒(CAV-2)F基因的核苷酸序列,用PCR或RT-PCR方法扩增克隆目的基因,分别构建了pIRCDV-H,pIRESVP1和pcCAV-F 3个真核表达质粒并进行了序列验证,用3种免疫质粒经纯化后混合对15只犬进行免疫,所有接种混合基因疫苗的犬,2次免疫后即可产生较高的抗体水平,攻毒后大部分犬能抵抗3种强毒的攻击,对照犬发病严重,证明所构建的核酸疫苗在一定程度上可抵抗强毒的攻击.

Abstract: The canine distemper virus,canine parvovirus,canine adenovirus are three kinds of diseases of canine.The CDV H gene,CPV VP1 gene,CAV F gene were clonded by PCR or RT-PCR.Recombinant plasmid pIRCDV-H,pIRESVP1,pcCAVF were constructed and sequenced to confirm its rightness.Fifteen dogs were injected at 9 weeks of age with three kinds plasmid of pIRCDV-H,pIRESVP1,pcCAV-F.All dogs were challenged with virulent CDV,CPV,CAV after the third injection.NAV vaccinated dogs showed a high IgG titer after the second injection and were protected against infection,while the control dogs were fully susceptible.That vaccines can protect some dogs against virulent was demonstrated.