Abstract: Comparative molecular field analysis(CoMFA)was performed to study 3D-QSAR of(E)-4-cinnamoyloxy-3-methoxystyrene derivatives with biological activity in order to give a theoretical basis to design novel more effective enzyme dual inhibitor.In this analysis,the cross validated coefficient R2CV was found to be 0.639,non-cross validated coefficient r2 and the standard deviation SEE was 0.744 and 0.148 respectively,and F was 198.355.The CoMFA models for twenty four of(E)-4-cinnamoyloxy-3-methoxystyrene derivatives showed the good relationship between steric and electrostatic properties with anti-inflammatory activity,which were very helpful for designing novel compounds as 5-lipoxygenase/cyclooxygenase inhibitor.