Abstract: The methods of electrically stimulated carotid thrombosis and inferior vein ligation in rats were used to evaluate the antithrombotic effects of polydatin.Rosette assay and Born’s method were used to observe plateletneutrophil interactions.The results showed that polydatin obviously protected against thrombosis caused by electrical stimulation and inferior vein ligation in rats.Polydatin also decreased the binding of thrombin-stimulated platelets to neutrophils and significantly suppressed platelet aggregation stimulated by N-formyl-methiongl-leucyl-phenylalanine-activated neutrophil suspension.The IC50 values were 51.9μmol/L and 307.6μmol/L,respectively.It is suggested that polydatin had evident antithrombotic effects and the mechanisms may be intimately related to suppression of platelet-neutrophil interactions.