Abstract: The chemical constituents of volatile oils from the Stenoloma chusanum(L.) Ching were analyzed and test was for their antibacterial activity.The volatile oils were obtained by steam distillation,its chemical components were determined by GC/MS analysis,and its antibacterial activity was carried out with paper scrip method.36 components were checked out and 24 compounds were identified,in which the main ingredients were L-linalool(24.76%),α-terpineol(7.24%) and geraniol(6.06%).The test for the antibacterial activity showed that volatile oils had obvious inhibition to Bacillus subtilis and Salmonella typhi,and had little inhibitory while almost no inhibition activity to Psedomonas aeruginose,Escherichia cdi,Staphylococcus aureus and Sarcina lutea.The main constituents in the volatile oils of Stenoloma chusanum(L.) Ching were monoterpene and 72.0 percent of them was identified.It was presumed that volatile oils from this plant contain some antibacterial constituents.