Abstract:
Three hybridomas secreting antibodies against specifically clenbuterol hydrochloride(CL),(β-adrenergic)agonist,which was diazotised and coupled to bovine serum albumin(BSA)to prepare immunogen,and to ovalbumin(OVA)to prepare coating conjugates,were produced by using hybridoma technology.By using competitive ELISA,the 50% inhibition concentration of free CL for 9E3,14C5 and 16F4 antibodies were 19.85,1.65?μg/L and 0.84?μg/L,respectively.The 16F4 antibody has approximately 0.26% cross reactivity to salbutamol,and approximately 0.04% to terbutalin,and no cross reactivity(0.02%)to fenoterol,adrenalin and noradrenalin.Therefore,these antibodies have high specificity for CL and could be used in immunological research and application.