Abstract: To find an optimal method to split influenza virus by Triton-X100,different concentrations of TritonX-100 were applied to split 3 different influenza virus,which were recommended by WHOas the 2007—2008 influenza vaccine production strains.The effect of split was observed by an electronic microscope.The immunity effect can detected after using the antigen that purified by sucrose density gradient centrifuge to immune the animal.It was showed that influenza Bcould be completely split by 1% Triton X-100,while influenza A-H1N1 and H3N2,could be completely split by 2% Triton X-100,which indicated that for different influenza strains,Triton X-100 performed different effect.Because the influenza vaccine production strains change every year,to insuring the quality of influenza ytic vaccine,it is necessary to investigate the different split concentrations for newly added strains,.