Abstract: Forty-seven phyllosphere fungal strains were isolated from 12 populations of an invasive plant Eupatorium adenophorum and 8 other plants in Yunnan and Guangxi provinces of China.Based on the characters of their colonies,hyphas and spore morphology,as well as the analysis of 18SrRNA gene restriction fragment length polymorphism(RFLP) and DNA sequencing,the strains were divided into 19 groups,phylogenetically close to Aspergillus niger,Cladosporium cladosporioides,Sporisorium reilianum,Colletotrichum lupine,Alternaria sp.,Sporobolomyces roseus,Dothideomycete sp.,Phoma sp.,Filobasidium elegans,Aureobasidium pullulans,Rhodotorula slooffiae,R.glutinis,Penicillium decumbens,Pestalotiopsis maculans,Cryptococcus aureus,Bimuria novae-zelandiae,Xylariaceae sp..Only 5 strains,which clustered together with Myrothecium sp.,C.lupine,Alternaria sp.,C.cladosporioides and Dothideomycete sp in the phylogenetic tree.,were pathogenic to the detached leaves of E.adenophorum.Two strains from R.glutinis were nonpathogenic to E.adenophorum but pathogenic to cabbages and potatoes.