袁志栋, 王志亮, 刘雨田, 吴晓东, 刘海生. 水貂朊蛋白前体基因的原核表达[J]. 云南大学学报(自然科学版), 2005, 27(2): 180-184.
引用本文: 袁志栋, 王志亮, 刘雨田, 吴晓东, 刘海生. 水貂朊蛋白前体基因的原核表达[J]. 云南大学学报(自然科学版), 2005, 27(2): 180-184.
shu
YUAN Zhi-dong, WANG Zhi-liang, LIU Yu-tian, WU Xiao-dong, LIU Hai-sheng. Cloning, prokaryotic expression of the mink PrP precursor gene[J]. Journal of Yunnan University: Natural Sciences Edition, 2005, 27(2): 180-184.
Citation: YUAN Zhi-dong, WANG Zhi-liang, LIU Yu-tian, WU Xiao-dong, LIU Hai-sheng. Cloning, prokaryotic expression of the mink PrP precursor gene[J]. Journal of Yunnan University: Natural Sciences Edition, 2005, 27(2): 180-184.
shu

水貂朊蛋白前体基因的原核表达

Cloning, prokaryotic expression of the mink PrP precursor gene

    摘要
  • 摘要: 构建了水貂朊蛋白前体基因的表达载体,所筛选出的阳性克隆质粒经转化表达菌BL21(DE3)后,收获表达菌,纯化鉴定表明获得了目的产物.

     

    Abstract: A recombinant plasmid designated as pET-32a-MPrP was constructed,which expresses mink PrP precursor fused protein.After transformed the positive recombinant plasmids to BL21 (DE3),the expression capacity of the fusion protein were optimized.After ultrasonicated,the sediment was isolated and purified.And the product was confirmed by Western Blot.

     

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