Abstract: The excessive accumulation of phenolic acids in tobacco root is an important reason for the occurrence of tobacco continuous cropping disorder. To alleviate the barriers to tobacco continuous cropping, a phenol acid-degrading strain N8 was isolated from the soil of continuous cropping tobacco field in Kunming, Yunnan Province. The strain was identified as Brevundimonas naejangsanensis by morphological and molecular biological methods. This strain could degrade 73.4% of ferulic acid and 83% of benzoic acid in tobacco continuous cropping soil. Compared with control group cultiveated in continuous croping soil in pot, plant height, stem circumference, and leaf length of tobacco treated by N8 strain increased by 17.22%, 16.86%, and 5.05%. In the field plot experiment, plant height and dry weight of tobacco in the N8 treatment group were higher than those in the CK control group, increased by 2.13% and 23.33%, respectively. Strain N8 screened in this study provided a beneficial strain reserve for eliminating tobacco continuous cropping barriers.