Abstract: It is crucial to establish an ecological security framework tailored to the current context to uphold ecological security, enhance the quality of the ecological environment, and propel sustainable development. In this study, Xishuangbanna was chosen as the focal area. Fourteen indices were selected based on the factors of water conservation, soil conservation, biodiversity conservation, and ecological sensitivity. The Analytic Hierarchy Process (AHP) was employed to calculate the weight of each factor. The Grey Relational Analysis (GRA) model was applied for single-factor evaluation, and a weighted combination of factors was executed in the raster calculator of ArcMap. Subsequently, the ecological security evaluation for the region was accomplished. Following this, the secure areas identified in the ecological security evaluation results served as ecological sources. The weighted superposition of the Normalized Difference Vegetation Index (NDVI) and Normalized Building Index (NDBI) was employed to construct the basic resistance surface. The Minimum Cumulative Resistance model (MCR) was used to extract ecological corridors and nodes, ultimately forming the comprehensive ecological security pattern for the study area. Finally, tailored ecological control measures were proposed based on the ecological security pattern and the land use status of the study area. The findings revealed that: (1) On the whole, the average ecological safety index for the study area is 0.571, indicating a general safety level, the safety level area constitutes 7.43%, relatively safe is 39.23%, general safety is 13.23%, forewarning is 23.64%, danger is 16.47%, and across different administrative divisions, all three counties (cities) exhibit general safety in their ecological security levels, with Jinghong (0.573) having a higher level compared to Mengla (0.571) and Menghai (0.568), the forewarning level proportion in Jinghong and Mengla both exceeds 27%; (2) The ecological source of the study area spans 7934.91 km², encompassing 132 ecological corridors with a total length of 2188.82 km and 53 ecological nodes; (3) The study area is categorized into three types of ecological function zones, ecological protection zones necessitate water and soil conservation and biodiversity preservation, ecological control zones require the regulation of construction land expansion and the provision of ecological products, production activity zones should engage in comprehensive environmental improvement to enhance the human living environment.