Abstract: Heterogeneous catalysts (CNM/Pd) supported by palladium nanoparticles on chitosan microspheres were prepared by sol-gel method and impregnation method. The catalysts were applied to hydrogenation of different quinolines and corresponding tetrahydroquinolines were synthesized. The effects of reaction time, temperature, amount of catalyst and type of solvent on the yield of template reaction were investigated by single factor control method. The results showed that under the conditions of 120 ℃, 0.25 mmol of quinoline, 3 mg of CNM/Pd, acetonitrile as solvent and reaction time for 6 h, the yield of the template reaction was the best, reaching 95.2%. It is proved that CNM/Pd has good reusability and substrate adaptability through catalyst recycling and substrate expansion.