Abstract: In order to determine the concentration of DNA in gene microarray and establish a method for quantification,we exchanged the bases between the sense and antisense strand and got different derived DNA,and used these derived DNA as the standard DNA for concentration curve establish.Since the complementary relatianship between templates and PCR primers were not changed,all derived DNA has same efficiency in the PCR amplification and hybridization,and can use it as standard DNA in making concentration curve.The results showed that the correlation coefficient between microarray signals and DNA copy number was more than 0.995.The test value was consistent with the expected concentration.This method provided a new thought for the research and development of the quantitative gene microarray.