Abstract:
Dgal,a gene encoding Gα,was firstly cloned from nematode-trapping fungus Drechslerella dactyloides by using methods of degenerate PCR and DNA Walking.The Dgal comprised 1 402 base pairs in length,and encoded a polypeptide with 356 amino acid residues.The theoretic molecular mass and pI of Dgal are deduced as 40.84 ku and 6.68,respectively.A phylogenetic analysis on fungal Gα indicates that the Dgal belongs to group Ⅲ of fungal Gα,which possibly has the function as stimulating the adenylyl cyclases.