Abstract: The allelopathy difference of three processing methods(the whole fresh sample,the crumble fresh sample and the sample which eliminated enzyme and dry by heat)of aqueous extracts of Eupatorium adenophorum on the germination of Eucalyptus globulus seeds was studied.The results indicated that the allelopathy effect tendency of three methods was similar.The multiple comparisons indicated that the difference of all testing items of thethree processing methods in high concentration were most obvious and the difference of thethree processing methods of sensitivetesting items in low concentration were most or more obvious.The multiple comparisons indicated the difference of all testing items of the processing methods between the crumble fresh sample and the sample which eliminated enzyme and dry by heat were not obvious.The function form and degree of allelopathy were bound up with sampletreatment means.The aqueous method of whole fresh sample was suggested becausethe method was the closest to natural leaching.