Abstract: The chemical constituents of Galinsoga parviflora were studied.Seven compounds were isolated from G.parviflora by column chromatogramphies of sephadex LH-20 and silica gel.Their structures were identified by physical and chemical properties and spectral data analysis.All the seven compounds were isolated from this plant for the first time.These compunds are:ent-kaur-16-en19-oicacid,ent-15-angeloyloxy-16-kauren-19-oic-acid,ent-15-angeloyloxy-16,17-epoxy-19-kauranoicacid,stigmasterol,α-spinasterol,α-spinasterylqoctadecanate,β-sitostel.Cytotoxicity screening of the extracts from Galinsoga parviflora Cav was carried out by HL60 cell.Two extractive fractions obtained with chloroform and ethyl acetate possess anticancer activities.Their IC50 are 8.5,10.5μg/mL respectively.