Abstract:
The ultraviolet fingerprint combined with chemometrics methods were used to analyze the Dendrobium samples and to set up a rapid method for identification of Dendrobium candidum which collected from different origins.It could be used the single factor experiment to determine the extraction conditions of the characteristic components of Dendrobium candidum.The optimal sample weight and the best extraction time were found to be 0.200 0 g and 30 min respectively.Dendrobium samples were extracted with petroleum ether,chloroform and ethanol and the extracting solutions were determined by UV spectra and then UV fingerprint of Dendrobium candidum was set up which were from different origins.The results showed that,Dendrobium candidum extracted by petroleum ether,chloroform and ethanol,in 24 h,the RSDs of stability were 00.99%,01.79%, 00.52%,the RSDs of accuracy were 00.04%,00.04%,00.09% and the RSDs of repeatability were 00.34%,00.30%,0.03%0.13% respectively.It showed that the method was stable and reliable.The analyses of the data have been performed by partial least squares discriminant analysis.The results showed that,the two-dimensional projection of the principal component scores can accurately distinguish Dendrobium samples from 5 areas.This method can be easily and quickly to identify the Dendrobium candidum which were from different regions and belong to the same provenance,and it could provide reference for identification of origins of other species.