Abstract: The chemical constituents from the branches and leaves of Pittosporum kerrii Craib were studied.Eight compounds were isolated from this plant by silica column chromatography and recrystallization.Their structures were identified by spectral data analysis.These are:2-nonacosanone(1),nonacosan-1-ol(2),ceryl alcohol(3),lacceroic acid(4),melissic acid(5),octacosanic acid(6),palmitic acid(7),(2S,3S,4R,8E)-2-(2R)-2 -hydroxytricosanoylamino-8-octadecene-1,3,4-triol(8).All these compounds were isolated for the first time from this plant.Compound 8 was isolated for the first time from Pittosporum.Cytotoxicity screening of extracts and the eight of compounds from P.kerrii Craib was carried out by K562 cell.Two compounds( 7,8 ) possess cytotoxic activities and the IC50 are 10.0 and 15.0 μg/mL for K562,respectively.