Abstract: Amylase, as an enzyme for hydrolyzing starch and glycogen, plays an important role in metabolism and industrial production. A strain of high-temperature amylase-producing bacteria was isolated and screened from the sediment of Shijiaquan hot spring in Midu County, Dali City, Yunnan Province. Its morphological, physiological and biochemical characteristics, 16S rRNA gene sequence analysis were carried out. The strain was identified as a strain of Bacillus sp., and named as Bacillus sp. Amy-43. The growth conditions and enzymatic properties of this strain were studied. The results showed that Bacillus sp. Amy-43 was resistant to high temperature and could still grow at 55 °C. The optimum growth temperature was 37 °C. The optimum enzyme activity temperature for amylase production was 75 °C, and the optimum reaction pH value was 4.0. Zn²⁺ and Mn²⁺ had a certain inhibitory effect on the enzyme. Ca²⁺, K⁺, Mg²⁺, Fe²⁺, Cu²⁺ all played a role in promoting the activity of the enzyme, of which Cu²⁺ was the most obvious. These characteristics indicate that the amylase from Bacillus sp. Amy-43 provides an important enzyme source for industrial application.