郑庆瑶, 熊晶晶, 程园园. Staphylococcus saprophyticus JJ-1协同所合成的钯纳米颗粒还原邻氯硝基苯[J]. 云南大学学报(自然科学版), 2020, 42(1): 149-155. doi: 10.7540/j.ynu.20190389
引用本文: 郑庆瑶, 熊晶晶, 程园园. Staphylococcus saprophyticus JJ-1协同所合成的钯纳米颗粒还原邻氯硝基苯[J]. 云南大学学报(自然科学版), 2020, 42(1): 149-155. doi: 10.7540/j.ynu.20190389
ZHENG Qing-yao, XIONG Jing-jing, CHENG Yuan-yuan. A composite system of Staphylococcus saprophyticus JJ-1 and its synthesized Pd nanoparticles for the reduction of o-chloronitrobenzene[J]. Journal of Yunnan University: Natural Sciences Edition, 2020, 42(1): 149-155. DOI: 10.7540/j.ynu.20190389
Citation: ZHENG Qing-yao, XIONG Jing-jing, CHENG Yuan-yuan. A composite system of Staphylococcus saprophyticus JJ-1 and its synthesized Pd nanoparticles for the reduction of o-chloronitrobenzene[J]. Journal of Yunnan University: Natural Sciences Edition, 2020, 42(1): 149-155. DOI: 10.7540/j.ynu.20190389

Staphylococcus saprophyticus JJ-1协同所合成的钯纳米颗粒还原邻氯硝基苯

A composite system of Staphylococcus saprophyticus JJ-1 and its synthesized Pd nanoparticles for the reduction of o-chloronitrobenzene

  • 摘要: 研究细菌合成纳米颗粒原位还原硝基苯类污染物,为废水中有机物的去除提供参考和思路. 首先利用Staphylococcu. saprophyticus JJ-1还原氯钯酸钠合成生物钯纳米颗粒(Bio-Pd NPs),采用一系列表征技术对所合成的Bio-Pd 纳米颗粒的形貌、尺寸、晶型进行分析. 进一步,利用“S. saprophyticus JJ-1/Bio-Pd”复合体系,还原转化邻氯硝基苯(O-Chloronitrobenzene,2-CNB),利用气相色谱-质谱联用仪(GC-MS)和高效液相色谱仪(HPLC)对2-CNB的还原途径和还原产物进行分析. 结果表明,S. saprophyticus JJ-1能够合成 Bio-Pd NPs,并于所合成的Bio-Pd NPs协同作用催化邻氯硝基苯的还原. GC-MS分析显示,邻氯硝基苯的还原产物为邻氯苯胺、硝基苯以及苯胺,说明在此体系下既可以实现硝基还原又可以进行脱氯反应. HPLC对邻氯硝基苯及其还原产物的定量分析显示,邻氯苯胺、硝基苯为中间产物,苯胺为最终产物. 此外,研究结果表明,为建立还原2-CNB的“S. saprophyticus JJ-1/Bio-Pd”复合体系,最适的氯钯酸钠浓度为0.2 mmol/L,邻氯硝基苯的浓度上限不应超过0.6 mmol/L. S. saprophyticus JJ-1能够快速的合成Bio-Pd NPs,并于所合成的Bio-Pd NPs构成协同体系,很好地催化邻氯硝基苯(2-CNB)的还原转化.

     

    Abstract: Reduction of nitrobenzene by bacteria and its synthesized nanoparticles provides a reliable method for treating organic pollutants in wastewater. Bio-Pd nanoparticles were synthesized by the reduction of sodium chloropalladate using S. saprophyticus JJ-1. The morphology, size and crystal form of the synthesized Bio-Pd nanoparticles were analyzed by a series of characterization techniques. o-chloronitrobenzene (2-CNB) was reduced using a composite system of S. saprophyticus JJ-1 and its synthesized Bio-Pd nanoparticles, and the reduction products and reduction pathways were analyzed. The results showed that S. saprophyticus JJ-1 reduced the palladium salt quickly and formed Bio-Pd nanoparticles subsequently. The “S. Saprophyticus JJ-1/Bio-Pd” system demonstrated excellent catalytic reduction performance for o-chloronitrobenzene. Gas chromatography-mass spectrometry (GC-MS) analysis showed that the reduction products of o-chloronitrobenzene were o-chloroaniline, nitrobenzene and aniline, indicating that the system can achieve nitro reduction and dechlorination simultaneously. Quantitative analysis of o-chloronitrobenzene and its products by high performance liquid chromatography (HPLC) showed that o-chloroaniline and nitrobenzene were intermediate products, and aniline was the final product. In addition, results showed that the optimal concentration of sodium chloropalladate in this system is 0.2 mmol/L, and the upper limit of o-chloronitrobenzene should not exceed 0.6 mmol/L. S. saprophyticus JJ-1 can rapidly synthesize Bio-Pd NPs, and has good reduction and conversion effects on o-chloronitrobenzene (2-CNB) under a synergistic catalytic system.

     

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