王鹏. 基于碳纳米线圈的细胞应激性测试探针[J]. 云南大学学报(自然科学版), 2020, 42(5): 941-948. doi: 10.7540/j.ynu.20200047
引用本文: 王鹏. 基于碳纳米线圈的细胞应激性测试探针[J]. 云南大学学报(自然科学版), 2020, 42(5): 941-948. doi: 10.7540/j.ynu.20200047
WANG Peng. Carbon nanocoil-based bioprobe for investigating cellular irritability[J]. Journal of Yunnan University: Natural Sciences Edition, 2020, 42(5): 941-948. DOI: 10.7540/j.ynu.20200047
Citation: WANG Peng. Carbon nanocoil-based bioprobe for investigating cellular irritability[J]. Journal of Yunnan University: Natural Sciences Edition, 2020, 42(5): 941-948. DOI: 10.7540/j.ynu.20200047

基于碳纳米线圈的细胞应激性测试探针

Carbon nanocoil-based bioprobe for investigating cellular irritability

  • 摘要: 外界力、电刺激对细胞行为具有显著影响,细胞的力、电应激特性研究广受关注. 使用碳纳米线圈对单个活体细胞定量施加局域力学和电学刺激,探究了细胞的应激特性. 研究发现碳纳米线圈的局域力刺激可以引起细胞的整体响应,且受激响应程度与外力的作用形式和大小有关. 另外,细胞在碳纳米线圈施加的局域电刺激下会产生显著极性响应,并可在撤掉电刺激后逐渐恢复至初始状态,表明其生理结构和功能未受到破坏. 基于碳纳米线圈的非侵入性柔性生物探针具有安全可控、易操作和低成本等优点,在活体细胞对的应激和传导机制研究、细胞行为调控等领域具有广阔的应用前景.

     

    Abstract: Mechanical and electrical stimulations play important roles on the cellular dynamic behavior, thus the mechanical and electrical responses of cells have been widely concerned. In this paper, for the investigation of cellular physiological characteristics, carbon nanocoils (CNCs) are used to exert local mechanical and electrical stimulation on a single living cell, for the first time. It is found that the local force stimulation of CNC causes global responses of cells, and the responses are related to the direction and magnitude of external forces. In addition, the cells show notable polarized responses under the local electrical stimulation from CNC−based electrodes. After removing the local electrical stimulation, the stimulated cells gradually return to the initial state, which indicates that the physiological structure and function of stimulated cells are not damaged. Above results show that CNC−based flexible bioprobes have many advantages of safety, controllability, easy operation and low cost. Thus, CNC−based probe is a powerful tool for the investigations for intracellular mechanical and electrical transduction mechanism and cellular behaviors regulation.

     

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