Abstract:
To verify the method of HPLC-MS/MS determination of notoginsenoside R
1, Ginsenoside Rg
1, Ginsenoside Rb
1 in plasma of SD rats, the pharmacokinetics of
Panax notoginseng extract in SD rats was studied. SD rats intragastrically administered
Panax notoginseng extract of 1500 mg/kg had their blood collected for the determination of plasma concentration by HPLC-MS /MS, after which pharmacokinetic parameters were calculated by DAS software. The established HPLC-MS/MS method has good specificity, and the correlation coefficients of the three consecutive batches of standard curves are all greater than 0.99 (weighted 1/
X2). The linearity of each component in certain concentration ranges was good. The RSD day precision of three components in plasma were less than 10% and the accuracy ranged from 88% to 105%. The diurnal precision (RSD) was less than 15% and the accuracy was between 93% to 109%. The stability of freeze-thaw and room temperature was good. The matrix effect does not affect the measurement effect. The non-compartmental model was used to calculate the pharmacokinetic parameters of Rg
1, Rb
1 and R
1 in rats. The results showed that the average values of AUC
0-t,
ρmax,
t1/2, and MRT
0-t, Rb
1>>Rg
1>R
1; average
Tmax value of Rb
1>> R
1> Rg
1. The established HPLC-MS/MS method is suitable for the pharmacokinetic study of Rg
1, Rb
1 and R
1 in
Panax notoginseng extract in rats. Rg
1 and R
1 have similar pharmacokinetic characteristics in rats, with rapid absorption and fast elimination; Rb
1 pharmacokinetic characteristics in rats are very different from Rg
1 and R
1, with slow absorption and slow elimination; Rb
1 exposure inplasma of rats take the absolute advantage.