Abstract:
Ophiocordyceps bispora was a species of the genus
Ophiocordyceps. Abiotic factors and microorganisms play important roles in the growth, development and adaptation of
Ophiocordyceps bispora.In this study, five group samples of
Ophiocordyceps bispora were sequenced by region (ITS2) Illumina MiSeq high-throughput sequencing technology, in order to compare fungal diversity, richness and community structure under different altitude gradients and light intensities. A total of 445 OTUs were gained, and 3 phyla, 13 classes, 41 orders, 69 families, 66 genera and 257 fungal species were identified. Ascomycetes was the dominant phylum and
Ophiocordyceps was the dominant genus. The correlation analysis between Alpha diversity index and altitude gradient showed that there were no significant correlation between fungal community diversity and richness and altitude gradient. Light intensity, vegetation type and human activities might be the main environmental factors affecting fungal community richness and diversity. The correlation analysis between fungal community and altitude gradient showed that 14 genera were positively correlated with altitude gradient. At the same time, Anosim analysis found that there were significant differences among the groups of
Ophiocordyceps bispora fungal communities. The above results showed that
Ophiocordyceps bispora contains rich in fungal resources. Altitude had a certain impact on
Ophiocordyceps bispora community structure, but has little impact on the richness and diversity of
Ophiocordyceps bispora. It is speculated that light intensities, human activities and vegetation types might be the main environmental factors affecting
Ophiocordyceps bispora fungal community structure, richness and diversity. This study provided a reference for the excavation and utilization of fungal resources in
Ophiocordyceps bispora microbial communities, and provided a research idea for the interaction between Cordyceps fungal communities and environment.