Abstract:
Formaldehyde in a new type of reconstituted tobacco for heating non-combustion cigarettes was determinated by continuous flow-spectrophotometer. Taking 0.5000 g of reconstituted tobacco (0.5 cm×0.5 cm) as sample, and 50 mL of 0.01 mol/L sodium hydroxide solution and 1.5 g activated carbon were added, the sample was vibrated for 1 h, filtered and the filtrate was collected. Continuous flow-the spectrophotometer was adopted, sodium formate was formed by the reaction of formaldehyde with sodium hydroxide, and the aldehyde group of sodium formate reacted with acetylacetone on-line to form an absorbable colour complex at 420 nm, the formaldehyde content in the reconstituted tobacco can be calculated. The results show that: ① The optimal pump tube configuration for the instrument is to use red/red for sample solution and deionized water and black/black for acetylacetone, the reaction mixing circle is 20 turns, and add a dialyzer to the analysis stream; ② The detection limit was 1.05 mg/kg, the quantitative limit was 3.50 mg/kg, the recovery was 95.5%~110.0% and the relative standard deviation was 0.18%~0.71%; ③ The method has the advantages of low cost, simple pretreatment, rapid determination, accurate quantification and low detection limit as compared with HPLC method, and is very suitable for the need of quality control of thin slice enterprises.