杜文文, 段青, 贾文杰, 马璐琳, 王祥宁, 王继华. 4种秋海棠花香挥发性物质测定与特征香气成分分析[J]. 云南大学学报(自然科学版), 2022, 44(5): 1043-1053. doi: 10.7540/j.ynu.20210455
引用本文: 杜文文, 段青, 贾文杰, 马璐琳, 王祥宁, 王继华. 4种秋海棠花香挥发性物质测定与特征香气成分分析[J]. 云南大学学报(自然科学版), 2022, 44(5): 1043-1053. doi: 10.7540/j.ynu.20210455
DU Wen-wen, DUAN Qing, JIA Wen-jie, MA Lu-lin, WANG Xiang-ning, WANG Ji-hua. Determination of volatile compounds and analysis of characteristic aroma components of four kind of Begonia species flowers[J]. Journal of Yunnan University: Natural Sciences Edition, 2022, 44(5): 1043-1053. DOI: 10.7540/j.ynu.20210455
Citation: DU Wen-wen, DUAN Qing, JIA Wen-jie, MA Lu-lin, WANG Xiang-ning, WANG Ji-hua. Determination of volatile compounds and analysis of characteristic aroma components of four kind of Begonia species flowers[J]. Journal of Yunnan University: Natural Sciences Edition, 2022, 44(5): 1043-1053. DOI: 10.7540/j.ynu.20210455

4种秋海棠花香挥发性物质测定与特征香气成分分析

Determination of volatile compounds and analysis of characteristic aroma components of four kind of Begonia species flowers

  • 摘要: 以4种秋海棠为试验材料,采用顶空固相微萃取(HS-SPME)与气相色谱−质谱(GC-MS)联用技术测定花香挥发性物质,并通过香气活性值(OAV)和主成分分析鉴定不同种类的特征香气和主要香气物质. 结果表明:①4个秋海棠种类中共检测到54种挥发性物质,萜烯类和苯类/苯丙素类化合物种类较为丰富,各种类花朵挥发性物质总含量不同,柳叶秋海棠最高,其次为中华秋海棠和假厚叶秋海棠,里氏秋海棠最低;②4种秋海棠中共鉴定出15种特征香气物质(OAV≥1),芳樟醇、β-月桂烯、D-柠檬烯、癸醛在不同种类中具有较高香气活性值,与各种类自身的特征香气物质一起形成各自独特香气,假厚叶秋海棠花朵更加突出果香气味,中华秋海棠花朵则更加突出草香气味,柳叶秋海棠花朵兼有果香、甜香气味,里氏秋海棠花朵则具有花香、果香和甜香气味;③各种类主要香气物质差异大,假厚叶秋海棠的主要香气物质为β-石竹烯,中华秋海棠的是α-法呢烯、β-罗勒烯、苯乙腈等,柳叶秋海棠中天竺葵醛、苯乙醇等贡献大,里氏秋海棠中则为二丁基羟基甲苯和十二醛.

     

    Abstract: Four kind of Begonia species were used as experimental materials. The volatile compounds in flower aroma were determined by headspace solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). Different characteristic aroma and main aroma compounds were identified by odar activity value (OAV) and principal component analysis. The results showed that: ① Fifty-four kinds of volatile compounds were detected in 4 species, the contents of terpenes and benzene/phenylpropanoid compounds are rich. The volatile substances and their total release of 4 species are different. The most volatile substances and total release are in B. fuchsioides , followed by B. grandis and B. pseudodryadis, and the lowest ones are in B. listada; ② There are 15 characteristic aroma substances (OAV ≥ 1), linalool, β-myrcene, D-limonene and decanal have high aroma activity values in 4 species, forming unique aroma together with their own characteristic aroma substances. The aroma characteristics of B. pseudodryadis flowers are mainly fruity ester- like flavor, green-like flavor is the most prominent in B. grandis, and B. fuchsioides is mainly mixed with fruity ester- like and sweet-like flavor; flora, fruity ester- like and sweet-like flavor are the most in B. listada; ③ The main aroma substance of B. pseudodryadis is caryophyllene, α-farnesene β-ocimene and benzyl nitrile, etc. is mainly in B. grandis, nonanal and phenylethyl alcohol in B. fuchsioides contribute greatly, while butylated hydroxytoluene and dodecanal has high contents in B. listada.

     

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