Abstract: The chemical constituents from Macaranga sampsonii were isolated and purified by semi-preparative high performance liquid chromatography, column chromatography on silica gel, Sephadex LH-20 and HPLC. The structures of the compounds were elucidated by NMR spectral analysis. Thirteen compounds were isolated and identified, including diosmetin (1), tangeretin (2) , nobiletin (3) , 5,4'-dihydroxy-3,6,7-trimethoxyflavone (4) , chrysosplenol D (5) , liquiritigenin (6) , epicatechin (7) , catechin (8) , isoliquiritigenin (9) , phloretin (10) , phloridzin (11) , trans-resveratrol-3-O-β-D-glucopyranoside (12) , magnolol (13) . All these compounds were first isolated from this plant. And compounds 4 and 13 showed weak anti-tumor activity.