基于谷胱甘肽刻蚀核壳Au@MnO2纳米粒子比色检测谷胱甘肽

Colorimetric detection of glutathione based on glutathione-induced etching of core-shell Au@MnO2

    摘要
  • 摘要: 谷胱甘肽（GSH）在控制细胞氧化还原状态中起着至关重要的作用，其在人体内浓度异常与许多疾病密切相关，因此，检测GSH具有重要意义. 在磷酸缓冲盐溶液（pH=6.2）中，核壳型Au@MnO2纳米粒子的外壳MnO2被GSH刻蚀，使体系溶液颜色改变（浅绿→浅红），吸光度降低. 基于此原理，通过测量Au@MnO2体系的比色信号随GSH浓度的变化，可以实现GSH的检测. 在优化的条件下，Au@MnO2传感体系检测GSH的线性范围为2nmol/L～0.1 mmol/L，检出限为1.62 nmol/L（S/N=3）. 该方法操作简单、灵敏度高、结果可视化，该传感体系对检测GSH具有很好的选择性，并可用于人血清样品中GSH的检测.

     

    Abstract: Glutathione (GSH) plays an important role in controlling the redox state of cells. The abnormal concentration of GSH is closely related to many diseases. Therefore, the detection of GSH is of great significance. In the phosphate buffer solution (pH=6.2), the MnO2 shell of core-shell Au@MnO2 nanoparticles was etched by GSH, which changed the color of the solution (light green→light red) and decreased the absorbance. Based on this principle, by measuring the colorimetric signals of Au@MnO2 with the change in GSH concentration, the colorimetric detection of GSH can be realized. Under the optimized conditions, the Au@MnO2 sensing system provided a linear range of 2 nmol/L−0.1 mmol/L with a low detection limit of 1.62 nmol/L (S/N=3). The method is simple, sensitive and visual. Moreover, the sensing system displayed a high selectivity for GSH detection, and can be used for the detection of GSH in human serum samples.

     

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