Abstract: The Panax notoginseng is an important Chinese medicinal material. Wenshan City is genuine producing areas for P. notoginseng. 92 samples and 45 soil and water samples were collected from 6 prefectures and cities in Kunming, Qujing, Baoshan, Chuxiong, Honghe and Wenshan Prefectures. The content of saponins, soil mineral elements and water factors of P. notoginseng from genuine producing area and new producing area were analyzed. The content of P. notoginseng saponins (Rb1, Rg1, R1) was determined by high performance liquid chromatography using GB/T 1908—2008. The methods GB 15618—2018 and GB 5084—2004 were used to detect soil mineral elements and water quality factors. The correlations and regression analyze between the content of total saponins and geographical location, mineral elements, water quality were analyzed. PCA analysis was carried out between the content of P. notoginseng saponins and soil mineral elements, water quality from different producing area. The results showed that there were a significant negative correlation between the contents of P. notoginseng saponins and altitude, north latitude for 2 years and 3 years old P. notoginseng, indicating that higher latitude and altitude were unfavourable for the accumulation of P. notoginseng saponins. The 13 components of soil and 7 components of water samples were not correlated with the accumulation of P. notoginseng saponins, indicating that planting soil and irrigation water had little effect on the content of P. notoginseng saponins. The research results interpreted the reason of P. notoginseng quality formation partly, and provides reliable and scientific basis for planting P. notoginseng in new producing area.