Abstract: Yunnan is the most seriously endangered province by Spodoptera frugiperda (Lepidotera: Noctuidae) in China, and a unified and coordinated control layout has important strategic significance for the prevention and control of Spodoptera frugiperda in Yunnan. In this paper, the idea of regional ecological prevention and control of S. frugiperda in Yunnan Province was put forward and comprehensively expounded. The main occurrence areas of S. frugiperda in Yunnan Province were divided to 7 regions based on the physical geographical features, the planting of host maize and biological characteristics of S. frugiperda. The regional ecological prevention and control measures were proposed and discussed, namely border control, pest source control and corridor control. This study will provide theoretical basis and scientific guidance for the prevention and control of S. frugiperda in Yunnan.