Abstract: The chemical constituents of the 95% ethanol extract from Cynodon dactylon (L.) Pers. were studied using silica gel, open ODS, Sephadex LH-20, and semi preparative HPLC column chromatography. The anti-inflammatory activities of isolated flavonoids and anthraquinones were studied. Sixteen compounds were isolated from bermudagrass and identified as tricin (1), chrysoeriol (2), isoliquiritigenin (3), tricin4′-O-(threo-β-guaiacyl-(7′′-O-methyl-9′′-O-acetyl)-glyceryl) ether (4), luteolin (5), tricin 4′-O-(erythro-β-guaiacylglyceryl) ether (6), calquiquelignan D (7), calquiquelignan E (8), 5,5′-dihydroxy-2′,4′-dim -ethoxyflavone-7-O-β-D-(6′′-O-Z-p-coumaroyl)-glucopyranoside (9), 4′-hydroxy-2,3-dihydroflavone-7-β-D-glucopyranoside (10), tricin 4′-O-(threo-β-guaiacyl-(7′′-O-methyl)-glyceryl) ether (11), ω-Hydroxyemodin (12), phomarin (13), aurantio-obtusin (14), β-sitosterol (15) and stigmasterol (16). Compounds 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 and 16 were isolated from this plant for the first time. Compounds 2, 3 and 14 showed significant anti-inflammatory activity, with IC₅₀ values of 2.74, 2.35 μmol/L and 3.61 μmol/L.