一株红树来源内生真菌Talaromyces assiutensis sp.活性次级代谢产物研究

Study on the secondary metabolites from mangrove-derived endophytic fungus Talaromyces assiutensis sp.

    摘要
  • 摘要: 为了从红树内生真菌中寻找具有农用活性的次级代谢产物，对一株来源于红树角果木内生真菌Talaromyces assiutensis sp.的活性次级代谢产物进行了研究. 综合运用各种色谱分离技术对内生真菌T. assiutensis sp.的发酵物进行分离纯化，采用1D/2D NMR、旋光以及高分辨质谱对纯化的次级代谢产物进行结构鉴定，共分离鉴定了15个次级代谢产物：5-乙酰氨基戊酸(1)，4-乙酰氨基丁酸甲酯(2)，pyrophen (3)， (E)-6-hydroxy-5-(1-propenyl)-1,2- dihydropyrano3,2-bpyrrole-3,7-dione (4)，N-乙酰基酪胺(5)，fonsecinone A (6)，aurasperone A (7)，asperpyrone D (8)，aurasperone F (9)，isoherqueinone (10)，ergosterdiacids B (11)，曲酸 (12)，L-1-柠檬酸甲酯 (13)，对羟基苯乙酸甲酯(14)和对羟基苯乙酸(15). 化合物1和2为首次报道1H/13C NMR数据的新天然产物. 首次对化合物1～15进行抗植物病原菌活性测试，发现聚酮类化合物6～10具有一定的抗真菌活性.

     

    Abstract: In order to search for anti-fungal secondary metabolites from endophytic fungi of mangroves, a mangrove-derived endophytic fungus Talaromyces assiutensis sp. from the mangrove Ceriops tagal was studied. Fifteen secondary metabolites (1−15) including 5-acetamidopentanoic acid (1), methyl-4-acetamidbutyrate (2), pyrophen (3), (E)-6-hydroxy-5- (1-propenyl)-1,2-dihydropyrano3,2-bpyrrole-3,7-dione (4), N-acetyltyramine (5), fonsecinone A (6), aurasperone A (7), asperpyrone D (8), aurasperone F (9), isoherqueinone (10), ergosterdiacids B (11), kojic acid (12), L-1-methyl citrate (13), methyl p-hydroxyphenylacetate (14) and p-hydroxyphenylacetic acid (15) were isolated from the T. assiutensis sp. by using modern chromatographic separation technology. Their structures were elucidated of the basis of spectroscopic and chemical data, especially NMR and MS. Compounds 1 and 2 are new natural products, which 1H/13C NMR data were reported for the first time. Compounds 1−15 were evaluated their anti-fungal activities, in which compound 6−10 showed anti-fungal activities.

     

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