番茄种质种子萌发期耐盐性综合评价及关键指标筛选

Comprehensive evaluation of salt tolerance during the germination period of tomato germplasm and Screening of key indicators

  • 摘要: 本研究旨在筛选番茄萌发期耐盐种质资源并确定关键评价指标. 首先采用6份番茄种质,设置0.0%(CK)、0.2%、0.4%、0.6%、0.8%、1.0%共6个NaCl浓度梯度,通过胚根长测定确定最适胁迫浓度;进而以20份番茄种质为材料,在最适浓度处理下测定8项指标(发芽率、发芽势、发芽指数、活力指数、胚根长、胚轴长及鲜重),计算耐盐系数后,综合运用相关性分析、主成分分析、隶属函数法和聚类分析进行评价,并通过回归分析筛选关键指标. 结果显示:(1)NaCl胁迫呈现"低促高抑"效应:0.2%-0.4%处理促进多数材料胚根伸长(20CL2045-0除外),0.6%显著抑制,0.8%-1.0%导致部分不萌发,确定0.6%为最适筛选浓度;(2)各指标耐盐系数显著相关(P<0.05),主成分分析提取2个主成分(累积贡献率78.114%);(3)聚类分析将材料分为高度耐盐型(2份)、中度耐盐型(15份)和敏感型(3份);(4)回归分析确定发芽指数、胚根长及鲜重为关键评价指标. 本研究建立了番茄萌发期耐盐评价体系,筛选出优异种质和关键指标,为耐盐机理研究和品种改良提供了重要依据.

     

    Abstract: This study aims to screen salt-tolerant germplasm resources during tomato germination and determine key evaluation indicators. First, 6 tomato germplasms were used, and 6 NaCl concentration gradients were set, 0.0% (CK), 0.2%, 0.4%, 0.6%, 0.8%, and 1.0%, and the optimal stress concentration was determined by radiculogenic length determination; then, 20 tomato germplasms were used as materials, and 8 indicators (germination rate, germination potential, germination index, vitality index, radiculogenic length, combyl length and fresh weight) were measured under the optimal concentration treatment. After calculating the salt tolerance coefficient, the correlation analysis, principal component analysis, membership function method and cluster analysis were comprehensively used for evaluation, and key indicators were screened through regression analysis. The results showed that: (1) NaCl stress showed a "low-promoting and high-inhibition" effect: 0.2%-0.4% treatment promoted the elongation of radiculogenic roots of most materials (except 20CL2045-0), with significant inhibition of 0.6%, and 0.8%-1.0% resulted in partial non-germination, and 0.6% was determined to be the optimal screening concentration; (2) The salt tolerance coefficients of each index were significantly correlated (P<0.05), and 2 principal components were extracted from principal component analysis (cumulative contribution rate 78.11%); (3) Cluster analysis divided the materials into highly salt-tolerant (2 parts), moderate salt-tolerant (15 parts) and sensitive (3 parts); (4) Regression analysis determined the germination index, radiculogenic length and fresh weight as key evaluation indicators. This study established a salt tolerance evaluation system for tomato germination period, screened out excellent germplasm and key indicators, providing an important basis for the study of salt tolerance mechanism and variety improvement.

     

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