云南妇女卵巢癌中p21WAF基因有较高的31位密码(Ser/Arg)突变

A high codon 31(Ser/Arg) mutation frequency of p21WAF in ovary tumor of Yunnan

摘要: 通过PCR-SSCP分析和序列分析,对取自云南地区的44例妇女卵巢肿瘤样本的p21WAF基因Ser31密码子的突变进行了分析.结果表明35例恶性肿瘤样本中有15例发现有突变,突变率为42.9%;9例良性肿瘤中有4例出现突变,突变频率44.4%;在另外6例正常组织中出现1例突变,突变率为16.7%.这一结果表明,云南妇女在这一基因位点存在多态性,这一位点的突变具有发生卵巢癌的趋势.

Abstract: Using the PCR-SSCP and sequencing technique,the codon 31(Ser/Arg) mutation of p21WAF of 36 ovary carcinomas samples were examined.The mutation was discovered in 41.6%(15 of 44) malignant ovary tumor and 50%(4 of 8) benign ovary tumor,only 16%(one of 6 ) normal ovary tissue.These results show that there is polymorphism at this location in Yunnan women,and this mutation tends to result in tumorigenesis.